In their first outing without their talisman Bryn Snellgrove, Douglas went down 33-25 away at Dukinfield.
The game was always likely to be a potential banana skin and saw Douglas slip away from the leading pack at the top of South Lancashire/Cheshire Division Two.
They are now fourth, just nine points clear of fifth placed Vagabonds.
Football: Island club wants community to design new crest and kit
Gymnasium AFC & Douglas Athletic FC merged during the summer
Boxing: Devine defeats Paredes to continue unbeaten run
Manxman moves onto 12 pro victories with unanimous points win
Athletics: Island athletes shine at British Masters
Seven-strong team claims 11 medals in Derby
Date confirmed for funeral of Sam Naughton
Isle of Man rider died following incident at this year's Manx Grand Prix
